> For the complete documentation index, see [llms.txt](https://kb.ezbiocloud.net/home/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://kb.ezbiocloud.net/home/protocols/16s-identification/get-started.md).

# Get Started

In this tutorial, we go over how to run 16S rRNA isolate samples through the 16S Identification tool.&#x20;

{% hint style="info" %}
16S rRNA isolates are bacterial species identified and isolated using the 16S rRNA gene as a molecular marker, and this method is fundamental for bacterial identification and taxonomy.
{% endhint %}

Let's identify your isolate using [your own amplicon data](/home/protocols/16s-identification/prepare-samples/private-samples.md) or practice with the [example files](/home/protocols/16s-identification/prepare-samples/public-samples.md), provided.

<table data-card-size="large" data-view="cards"><thead><tr><th></th><th></th><th></th><th data-hidden data-card-target data-type="content-ref"></th></tr></thead><tbody><tr><td><h3>Private Samples</h3></td><td>Locally stored 16S rRNA files in FASTA, AB1, or text format</td><td></td><td><a href="/pages/ELK98Lnj2m4qqDuAy9Zh">/pages/ELK98Lnj2m4qqDuAy9Zh</a></td></tr><tr><td><h3>Public Samples</h3></td><td>Retrieve public 16S rRNA files in FASTA, AB1, or text format</td><td></td><td><a href="/pages/ulOXu7EXtrTIoCcrvoFm">/pages/ulOXu7EXtrTIoCcrvoFm</a></td></tr></tbody></table>


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://kb.ezbiocloud.net/home/protocols/16s-identification/get-started.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
