Nanopore

Unassembled isolate genome sequences (long reads, single-end FASTQ files)

Select 'Nanopore'

Single-end (unassembled genome isolate)

For storing DNA sequencing reads from a nanopore sequencing run, where each read is represented by two or three lines of data, including the sequence, quality scores, and optionally, additional metadata.

Compressed

Several 16S sequences can be uploaded simultaneously as multiple text files or by a single multi-FASTA text file.

FASTA upload

Upload FASTA file samples

Upload your sample sequence files by dragging them into the ‘Upload’ box or by clicking the [+] icon and navigating to the directory of your sequences for upload.

Proceed with upload

After your files have been uploaded, you can proceed with processing each sample by selecting the blue ‘Upload’ box.

EzBioCloud takes it from here

EzBioCloud's 16S Identification tool will search its 16S database for the closest matches to each sample.

The status bar will indicate when your sample report is ready in no time.

Once your sample has been processed, it will remain under your 16S Identification list.

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Text upload

Batch Upload is also possible using text format.

Upload text format samples

Alternatively, you can directly upload sequences text by selecting ‘Upload in text’ format and inserting the sequences.

Paste multiple sequences

Copy and paste the 16S rRNA sequences text into the 'Sequence...' box in FASTA format text and select 'Next'.

Proceed with upload

Select the blue 'Upload' box.

Once your samples have been processed, they will remain under your 16S Identification list.

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Explore your sample report

Select the PDF option under the ‘Report’ column for an in-depth analysis of your sample. This includes species-to-strain identification, AMR genes, VIR factors, coverage, top hits (matches), and a phylogenetic tree