Prepare Samples
Last updated
Last updated
Uploading your 16S rRNA sequencing data to our private service is a straightforward process, but there are a few steps to ensure everything goes smoothly. First, we must prepare the samples for upload. There are three sample types to choose from.
Depending on the sequencing machine and settings used, samples will produce different types of files.
A commonly used text-based format for representing nucleotide or protein sequences, where each sequence is preceded by a single-line description starting with a '>' symbol.
A binary file format from Applied Biosystems DNA sequencers that stores DNA sequencing chromatogram data, including the raw signal intensity and quality scores for each base call.
In the style of FASTA format, the text of a 16S rRNA sequence can be directly uploaded.
Let's identify your isolate using your amplicon data or practice with the example files, provided.
Locally stored 16S rRNA files in FASTA, AB1, or text format
Retrieve public 16S rRNA files in FASTA, AB1, or text format
