# Single Upload

## FASTA upload

### Upload your FASTA file sample

Upload your sample’s sequence file by dragging it into the ‘Upload’ box or by clicking the \[+] icon and navigating to the directory of your sequences for upload.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FLKPxEkPlVskPzBARwED3%2Fimage.png?alt=media&#x26;token=62e01bc0-9fb8-46e8-b856-9997ededc0a0" alt=""><figcaption></figcaption></figure>

### Proceed with upload

After your file has been uploaded, you can proceed with processing each sample by selecting the blue ‘Upload’ box.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FJrKQusouaa7TeEioe4cY%2Fimage.png?alt=media&#x26;token=4b5e3714-5217-4e79-8145-f90275ce67a0" alt=""><figcaption><p>The credit cost will be displayed at the bottom left of the box.</p></figcaption></figure>

### EzBioCloud takes it from here

EzBioCloud's 16S Identification tool will search its 16S database for the closest matches to your sample. &#x20;

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2F2sIcVj4BXs8OibmLY3OW%2Fimage.png?alt=media&#x26;token=091bb3c6-08b6-44c6-a546-e7ef2f2850f6" alt=""><figcaption></figcaption></figure>

{% hint style="info" %}
The status bar will indicate when your sample report is ready in no time.&#x20;
{% endhint %}

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2Ffaz4wH0UcYPET6aZNnTK%2Fimage.png?alt=media&#x26;token=fbdb88e4-d552-47f1-acfa-4b16d9919d25" alt=""><figcaption></figcaption></figure>

Once your sample has been processed, it will remain under your 16S Identification list.&#x20;

***

## Text upload

### Upload your text format sample

Alternatively, you can directly upload the sequence text by selecting ‘Upload in text’ format and inserting the sequence.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FkISes0R3ZROBDkuiZO2o%2Fimage.png?alt=media&#x26;token=7f4a330a-376c-4d89-85c9-5c3734542b69" alt=""><figcaption></figcaption></figure>

Copy and paste the 16S rRNA text into the 'Sequence...' box and select the blue 'Next' box.&#x20;

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FaAd1lbY68ULppRIUbEyB%2Fimage.png?alt=media&#x26;token=8f87d13d-5035-4a2d-97cc-f3e2fafe2a3c" alt=""><figcaption><p>Here we copied the 16S rRNA text in the FASTA format style which begins with '>Identifier'. </p></figcaption></figure>

### Proceed with upload

After the 16S rRNA text has been uploaded, you can proceed with processing the sample by selecting the blue ‘Upload’ box.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FCXAXLWgNyYbxi7oxD3bh%2Fimage.png?alt=media&#x26;token=772c3ff2-62b9-48ac-88fd-7cd08a8cae39" alt=""><figcaption></figcaption></figure>

### EzBioCloud takes it from here

EzBioCloud's 16S Identification tool will search its 16S database for the closest matches to your sample. &#x20;

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FkSBS4l9G1pZ4FLZYJKKT%2Fimage.png?alt=media&#x26;token=d47a5d5d-4574-46bc-bf2f-60b6e03bd66a" alt=""><figcaption></figcaption></figure>

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2F6sT2PgCIqLI938NFyT0I%2Fimage.png?alt=media&#x26;token=7f4c4aab-c9fc-44b6-9448-c4b742e0637f" alt=""><figcaption></figcaption></figure>

Once your sample has been processed, it will remain under your 16S Identification list.&#x20;

***

## [Explore your sample report](https://kb.ezbiocloud.net/home/protocols/16s-identification/download-report)

Select the PDF option under the ‘Report’ column for an in-depth analysis of your sample. This includes species identification, coverage, top hits (matches), and a phylogenetic tree.