# Single Upload

## FASTA upload

### Upload your FASTA file sample

Upload your sample’s sequence file by dragging it into the ‘Upload’ box or by clicking the \[+] icon and navigating to the directory of your sequences for upload.

<figure><img src="/files/LDjTqDdonFvCKJDJH5RH" alt=""><figcaption></figcaption></figure>

### Proceed with upload

After your file has been uploaded, you can proceed with processing each sample by selecting the blue ‘Upload’ box.

<figure><img src="/files/tunqSW5gMu30yf3GqbZL" alt=""><figcaption><p>The credit cost will be displayed at the bottom left of the box.</p></figcaption></figure>

### EzBioCloud takes it from here

EzBioCloud's 16S Identification tool will search its 16S database for the closest matches to your sample. &#x20;

<figure><img src="/files/UDuyWJIVm0wbb4fSp8si" alt=""><figcaption></figcaption></figure>

{% hint style="info" %}
The status bar will indicate when your sample report is ready in no time.&#x20;
{% endhint %}

<figure><img src="/files/sn0ldc1citVU2UtharV5" alt=""><figcaption></figcaption></figure>

Once your sample has been processed, it will remain under your 16S Identification list.&#x20;

***

## Text upload

### Upload your text format sample

Alternatively, you can directly upload the sequence text by selecting ‘Upload in text’ format and inserting the sequence.

<figure><img src="/files/18TyK9UJ6Yr10jhC3mkL" alt=""><figcaption></figcaption></figure>

Copy and paste the 16S rRNA text into the 'Sequence...' box and select the blue 'Next' box.&#x20;

<figure><img src="/files/kchxLwILpeWlBqRU6Gxg" alt=""><figcaption><p>Here we copied the 16S rRNA text in the FASTA format style which begins with '>Identifier'. </p></figcaption></figure>

### Proceed with upload

After the 16S rRNA text has been uploaded, you can proceed with processing the sample by selecting the blue ‘Upload’ box.

<figure><img src="/files/cTjr0e9f1TBIRZhXdkFh" alt=""><figcaption></figcaption></figure>

### EzBioCloud takes it from here

EzBioCloud's 16S Identification tool will search its 16S database for the closest matches to your sample. &#x20;

<figure><img src="/files/zS43XrWY4I8qXV5wpb7N" alt=""><figcaption></figcaption></figure>

<figure><img src="/files/xkeoJyxBW5pOm7RV7yGp" alt=""><figcaption></figcaption></figure>

Once your sample has been processed, it will remain under your 16S Identification list.&#x20;

***

## [Explore your sample report](/home/protocols/16s-identification/download-report.md)

Select the PDF option under the ‘Report’ column for an in-depth analysis of your sample. This includes species identification, coverage, top hits (matches), and a phylogenetic tree.


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