> For the complete documentation index, see [llms.txt](https://kb.ezbiocloud.net/home/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://kb.ezbiocloud.net/home/protocols/shotgun-microbiome/create-datasets.md).

# Create Datasets

Now that we have profiled samples as part of a study and associated metadata, we want to create a dataset for analyses. To create a dataset, we attach the metadata to the matching profiles.&#x20;

## Navigate to the 'Dataset' Tab

<figure><img src="/files/MoU7PF4iFwLCxsyGlBnB" alt=""><figcaption></figcaption></figure>

## Create Dataset

Select 'Create a New Dataset' and fill in the basic information parameters.

<figure><img src="/files/y7I79OlxCEOEOqGNyHY6" alt=""><figcaption></figcaption></figure>

## Select Profiles for a Dataset

There are multiple ways to select profiles for a dataset. We will use the default upload type method. Either, click or drag and drop the concatenated .xlsx file from the metadata steps into the upload area.&#x20;

{% file src="/files/fAtG4EnggndZd16uI7Ug" %}
Example, concatenated metadata .xlsx file for dataset preparation.
{% endfile %}

{% hint style="info" %}
Datasets can be made up of any profiled samples in your account i.e. any sample with a profile\_id. These can be profiles from an entire study, a combination of studies, or individually selected profiles. The choice is up to you.&#x20;
{% endhint %}

<figure><img src="/files/Sp02oQDYpotbtMD9WyM9" alt=""><figcaption></figcaption></figure>

{% hint style="warning" %}
Double-check the sample name matches the run name, ensuring the metadata is correctly assigned to the right samples.
{% endhint %}

## Select 'Upload'

Upload the selected profiles to the dataset.&#x20;

<figure><img src="/files/kZV9T5wZAw1TneaHadZe" alt=""><figcaption><p>Processing the dataset</p></figcaption></figure>

<figure><img src="/files/Oy2ye51P5mVIemxMYEU5" alt=""><figcaption><p>Successful dataset upload</p></figcaption></figure>

## Explore Dataset

Clicking on the dataset will show you any analyses we have run on it and the metadata associated with each sample/run.&#x20;

<figure><img src="/files/TSpckjAmRnB4dp8Xitf1" alt=""><figcaption></figcaption></figure>

{% hint style="info" %}
Fields like Run, age\_at\_collection, etc. have all been added from the SRA. It’s good to confirm these are ready for the next step, analysis.
{% endhint %}

<figure><img src="/files/fFvbjEKLfwvE9mutkkNn" alt=""><figcaption></figcaption></figure>

Now let’s analyze this dataset!
