# Batch Upload

Several 16S sequences can be uploaded simultaneously as multiple text files or by a single multi-FASTA text file.

## FASTA upload

### Upload FASTA file samples <a href="#upload-your-fasta-file-sample" id="upload-your-fasta-file-sample"></a>

Upload your sample sequence files by dragging them into the ‘Upload’ box or by clicking the \[+] icon and navigating to the directory of your sequences for upload.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FEOJ2oUd3DIitXTruS6t0%2Fimage.png?alt=media&#x26;token=b610d8a6-d7b4-440a-aed7-a12774aaa096" alt=""><figcaption></figcaption></figure>

### Proceed with upload <a href="#proceed-with-upload" id="proceed-with-upload"></a>

After your files have been uploaded, you can proceed with processing each sample by selecting the blue ‘Upload’ box.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2F9yOz4jA1yVCCuKdTPlLP%2Fimage.png?alt=media&#x26;token=2b942ee3-79d4-4cd1-b981-93155770aa52" alt=""><figcaption></figcaption></figure>

### EzBioCloud takes it from here <a href="#ezbiocloud-takes-it-from-here" id="ezbiocloud-takes-it-from-here"></a>

EzBioCloud's 16S Identification tool will search its 16S database for the closest matches to each sample.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FCvyJimcki7TzsH1aNcAB%2Fimage.png?alt=media&#x26;token=912fdc55-48aa-4302-874d-d0153e1e14e0" alt=""><figcaption></figcaption></figure>

{% hint style="info" %}
The status bar will indicate when your sample report is ready in no time.
{% endhint %}

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2Fxmr7nx26WvHzGN8hcgFb%2Fimage.png?alt=media&#x26;token=e1168d65-0e3b-4398-a327-339b56280af5" alt=""><figcaption></figcaption></figure>

Once your sample has been processed, it will remain under your 16S Identification list.

***

## Text upload

Batch Upload is also possible using text format.&#x20;

### Upload text format samples <a href="#upload-your-text-format-sample" id="upload-your-text-format-sample"></a>

Alternatively, you can directly upload sequences text by selecting ‘Upload in text’ format and inserting the sequences.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2Fo4LdTzihFheA2g9YtHZ8%2Fimage.png?alt=media&#x26;token=5dca835e-548a-4ecf-a1df-e48b555cff8d" alt=""><figcaption></figcaption></figure>

### Paste multiple sequences

Copy and paste the 16S rRNA sequences text into the 'Sequence...' box in FASTA format text and select 'Next'.&#x20;

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FnQacdjIv9o24m6fZonnS%2Fimage.png?alt=media&#x26;token=70d90bb8-267c-4bb7-9176-e59c10f1cf86" alt=""><figcaption></figcaption></figure>

### Proceed with upload

Select the blue 'Upload' box.

<figure><img src="https://820779907-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FDWKOAVP0eaMhg1acSkor%2Fuploads%2FzhLHoiKHijjEnUBwQI14%2Fimage.png?alt=media&#x26;token=40cf08f3-f3cf-40c5-96d8-e2e480a73699" alt=""><figcaption></figcaption></figure>

Once your samples have been processed, they will remain under your 16S Identification list.&#x20;

***

## [Explore your sample report](https://kb.ezbiocloud.net/home/protocols/16s-identification/download-report)

Select the PDF option under the ‘Report’ column for an in-depth analysis of your sample. This includes species identification, coverage, top hits (matches), and a phylogenetic tree.